What is it?
Highly sensitive real time PCR kit
This assay kit allows researchers to detect KRAS and BRAF mutations in colorectal cancer patients which can hinder the effectiveness of typically expensive targeted therapy.
Highly sensitive and cost-effective, differentiates eight KRAS mutations found in codons 12 and 13 and one BRAF mutation in codon 600.
The kit can conduct 24 tests and delivers results three hours after extraction.
Who is it for?
This kit is especially valuable to colorectal cancer researchers.
Please note that the GenoScreen KRAS/BRAF real time PCR kit is for research purposes and molecular biology applications only.
This product is not intended for the diagnosis, prevention or treatment of disease.
Why is it different?
This highly sensitive kit allows selective site-specific detection of mutant DNA.
It can detect less than 0.1% mutant sequence copies against a background of 99.9% wild-type DNA.
The ready-to-use reagent is compatible with various real time PCR machines and delivers results 3 hours after extraction.
Accurate – Highly sensitive test allows site-specific detection of less than 0.1% mutant sequence copies in a background of 99.9% wild-type DNA
Fast – Delivers results in approximately 3 hours, tests up to 8 samples simultaneously
Easy – No post-PCR manipulation required
Cost effective – Lowest overall cost in market
Need to know
This is a consumable assay kit, developed, supplied and supported by DiagCor. It requires a compatible instrument and a meticulous MDx workflow.
What you get
In the box
Service and support
KRAS/BRAF PCR master mix
KRAS G12R primer mix
KRAS G12S primer mix
KRAS G12C primer mix
KRAS G12D primer mix
KRAS G12A primer mix
KRAS G12V primer mix
KRAS G13C primer mix
KRAS G13D primer mix
BRAF V600E primer mix
KRAS/BRAF control primer mix
DNase free water
The DiagCor support team includes skilled technicians, engineers and scientists
Distributor and end-users receive direct training supported by video conferencing
Standard operation procedures explain test execution step-by-step
Experienced technicians will familiarize users with procedures by guiding them through a series of trial tests
DiagCor provides extensive training to end users and distributors that covers
Correct application of the system, including how to deploy in public health programs for best ROI
Practical lab technician training
Real-Time PCR machine
High speed centrifuge
QIAamp FFPE extraction kit
KRAS mutation (8 mutations)
BRAF mutation (1 mutation)
||24 tests (real time PCR kit)
||Eight KRAS mutations in codon 12 and 13: G12R GGT > CGT, G12S GGT > AGT, G12C GGT > TGT G12D GGT > GAT G12A GGT > GCT G12V GGT > GTT, G13D GGC > GAC, G13C GGC > TGC, one BRAF mutation codon 600: V600E GTG > GAG
||Human genomic DNA extracted from fresh, frozen or paraffin embedded tissue
||Positive control; negative control
||Wild-type or mutated KRAS or BRAF