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GenoQuantTM Periodontal Bacteria qPCR Assay

The GenoQuantTM Periodontal Bacteria qPCR Assay is designed for qualitative and quantitative analysis of six common periodontal bacteria in human saliva.

What is it?

Periodontitis is a highly prevalent chronic inflammatory disease leading to the loss of periodontal tissues, which is the major cause of tooth loss in adults. Several periodontal bacteria have been identified to be responsible for the disease and may relevant to other systemic infectious diseases including meningitis, osteomyelitis, and arthritis. Each group of bacteria plays a significant different role in the pathogenesis of periodontitis.

 

This assay provides not only direct evidence on the presence of microbes, but also the bacteria count (copies/μL) of six common periodontal bacteria (Aggregatibacter actinomycetemcomitans (AA), Porphyromonas gingivalis (PG), Tannerella forsythensis (TF), Treponema denticola (TD), Prevotella intermedia (PI), and Fusobacterium nucleatum (FN)).

Who is it for?

This assay is intended exclusively for in vitro diagnostic use with the appropriate clinical laboratory settings, safety standards and properly trained personnel.

 

The information provided by the test is extremely valuable for practitioners to select the appropriate antibiotics in periodontitis treatment. With the provided therapeutic threshold for each bacterium, dental healthcare professionals can provide personalized antibiotics treatment to the patient and evaluate the effectiveness of treatment.

Why is it different?

This kit provides a qualiitative and quantitative analysis of the most common periodontal bacteria.
 
The ready-to-use reagent is compatible with various real time PCR machines and the whole process may be completed in less than 2.5 hours.

Advantages

Accurate – Highly sensitive, qualitative and quantitative test
Practical – Compatible with various real time PCR instruments
Fast – Results in 2.5 hours
Easy – Ready-to-use reagent
Cost effective – Highly price-competitive

Need to know

This is a consumable assay kit, developed, supplied and supported by DiagCor. It requires a compatible instrument and a meticulous MDx workflow.

  • Works with

    Genesis 96Q Real-Time PCR System

    Genesis 96Q Real-Time PCR System is a compact 96-well real-time PCR (qPCR) instrument with up to 6 detection channels to satisfy various analysis methods.

    Applied Biosystems ViiA7

  • Workflow
    Workflow (GenoScreen & GenoQuant)

What you get

In the box

  • Perio. Bact. PCR Master Mix
  • AA Primer Mix
  • PG Primer Mix
  • TF Primer Mix
  • TD Primer Mix
  • PI Primer Mix
  • FN Primer Mix
  • Perio. Bact. Positive Standard 1
  • Perio. Bact. Positive Standard 2
  • Negative Control
  • DNase Free Water

Service and support
The DiagCor support team includes skilled technicians, engineers and scientists
 
  • Distributor and end-users receive direct training supported by video conferencing
  • Standard operation procedures explain test execution step-by-step 
  • Experienced technicians will familiarize users with procedures by guiding them through a series of trial tests 

Equipment required
  • Real-Time PCR machine
  • High speed centrifuge
  • Vortex
  • Pipettes
  • Water bath
  • Ice bucket 

Analytes
  • hbpA gene of AA
  • rpoB gene of PI
  • hsp60 gene of TF
  • cfpA gene of TD
  • fadA gene of FN
  • rpoB gene of PG
  • RPP gene of AC

Specs
Catalogue number 92030
Configuration 24 tests (real time PCR assay)
Detected genes hbpA gene of AA; rpoB gene of PI; hsp60 gene of TF; cfpA gene of TD; fadA gene of FN; rpoB gene of PG; RPP gene of AC
Specimens Human saliva
Internal controls PS1, PS2, NC, AC
Reported results AA/PI/TF/TD/FN/PG/AC positive/negative & therapeutic threshold