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GenoScreenTM SARS-CoV-2 RT-qPCR Kit

SARS-CoV-2 RT-qPCR kit is designed for the detection of SARS-like coronaviruses in human respiratory specimens via real time PCR analysis. It is a triplex assay that detects two viral genes (ORF1ab and N) and an internal control.

What is it?

Accurate detection of SARS-CoV-2
 
This sensitive, highly specific kit works with various real time PCR machines to quickly detect SARS-CoV-2 in clinical specimens. 
 
It is a triplex assay that detects two viral genes (ORF1ab and N) and an internal control using specific primers and fluorescent dye-labeled oligonucleotide probes. These genes are amplified and detected in commonly used FAM, HEX/VIC and Cy5 fluorescent channels, respectively.
 
The cost-effective ready-to-use reagent produces results two hours after extraction.

Who is it for?

The kit is especially valuable for the research of SARS-CoV-2.
 
Please be noted that the GenoScreen SARS-CoV-2 RT-qPCR kit is for research purpose and molecular biology applications only. 
 
This product is not intended for the clinical diagnosis, prevention  or treatment of COVID-19.  

Why is it different?

The design is adapted from well established HKU-Pasteur protocol with further improvement, simplifying the master mix preparation step from two monoplex assays to a single triplex assay with the combination use of highly thermostable enzymes.  
 
The ready-to-use reagent is compatible with various real time PCR machines and delivers results 2 hours after extraction.

Advantages

Fast – Delivers results in approximately 2 hours, tests up to 96 samples simultaneously
Accurate – Reliable ready-to-use reagent
Cost effective – Lowest overall cost in market
Easy – No post-PCR manipulation required

Need to know

This is a consumable assay kit, developed, supplied and supported by DiagCor. It requires a compatible instrument and a meticulous MDx workflow.

  • Workflow
    Workflow (GenoScreen & GenoQuant)

What you get

In the box

  • 5x master mix
  • 40x RT mix
  • SARS-CoV-2 primer mix
  • Magnesium chloride
  • SARS-CoV-2 positive control
  • Negative control 
  • Nuclease-free water
 

Service and support
The DiagCor support team includes skilled technicians, engineers and scientists
 
  • Distributor and end-users receive direct training supported by video conferencing
  • Standard operation procedures explain test execution step-by-step 
  • Experienced technicians will familiarize users with procedures by guiding them through a series of trial tests 
 
DiagCor provides extensive training to end users and distributors that covers
 
  • Correct application of the system, including how to deploy in public health programs for best ROI
  • Practical lab technician training    

Equipment required
  • Real-time PCR machine
  • Centrifuge
  • Vortex
  • Pipettes
  • Water bath
  • Ice bucket 

Extraction kits

QIAamp Viral RNA Mini Kit (QIAGEN, Germany)


Analytes
Internal amplification control (IAC)
SARS-CoV-2 (ORF1ab and N genes)

Specs
Catalogue number R20002
Configuration 48 tests (real time PCR kit)
Detected genes ORF1ab and N genes of SARS-CoV-2
Analytical sensitivity ≥ 10 copies/rxn
Specimens Human respiratory tract specimens
Internal controls Positive control; negative control; Internal amplification control (IAC)
Reported results SARS-CoV-2 positive or negative