¿Qué es?
Accurate detection of SARS-CoV-2
This sensitive, highly specific kit works with various real time PCR machines to quickly detect SARS-CoV-2 in clinical specimens.
It is a triplex assay that detects two viral genes (ORF1ab and N) and an internal control using specific primers and fluorescent dye-labeled oligonucleotide probes. These genes are amplified and detected in commonly used FAM, HEX/VIC and Cy5 fluorescent channels, respectively.
The cost-effective ready-to-use reagent produces results two hours after extraction.
¿Para quién es?
This assay is intended exclusively for in vitro diagnostic use with the appropriate clinical laboratory settings, safety standards and properly trained personnel.
It is for SARS-like coronaviruses RNA detection and has been validated using human respiratory samples (e.g. deep throat saliva) extracted by a CE IVD extraction system and designated PCR platform.
¿Por qué es diferente?
The design is adapted from well established HKU-Pasteur protocol with further improvement, simplifying the master mix preparation step from two monoplex assays to a single triplex assay with the combination use of highly thermostable enzymes.
The ready-to-use reagent is compatible with various real time PCR machines and delivers results 2 hours after extraction.
Ventajas
Rápido – Delivers results in approximately 2 hours, tests up to 96 samples simultaneously
Exacto – Reliable ready-to-use reagent
Costo-efectivo – Lowest overall cost in market
Fácil – No post-PCR manipulation required
Más detalles
This is a consumable assay kit, developed, supplied and supported by DiagCor. It requires a compatible instrument and a meticulous MDx workflow.
- Funciona con
Genesis 96Q Real-Time PCR System is a compact 96-well real-time PCR (qPCR) instrument with up to 6 detection channels to satisfy various analysis methods.
- Flujo de trabajo
¿Qué viene en la caja?
En la caja encontrará
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5x master mix
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40x RT mix
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SARS-CoV-2 primer mix
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Magnesium chloride
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SARS-CoV-2 positive control
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Negative control
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Nuclease-free water
Servicio y soporte
The DiagCor support team includes skilled technicians, engineers and scientists
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Distributor and end-users receive direct training supported by video conferencing
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Standard operation procedures explain test execution step-by-step
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Experienced technicians will familiarize users with procedures by guiding them through a series of trial tests
DiagCor provides extensive training to end users and distributors that covers
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Correct application of the system, including how to deploy in public health programs for best ROI
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Practical lab technician training
Equipos requeridos
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Real-time PCR machine
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Centrifuge
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Vortex
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Pipettes
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Water bath
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Ice bucket
Kits de extracción
QIAamp Viral RNA Mini Kit (QIAGEN, Germany)
Cobertura
Internal amplification control (IAC)
SARS-CoV-2 (ORF1ab and N genes)
Especificaciones
| Número de catálogo |
92027 |
| Configuración |
48 tests (real time PCR kit) |
| Genes detectados |
ORF1ab and N genes of SARS-CoV-2 |
| Sensibilidad analítica |
≥ 10 copies/rxn |
| Tipo de muestra |
Human respiratory tract specimens |
| Controles internos |
Positive control; negative control; Internal amplification control (IAC) |
| Resultados reportados |
SARS-CoV-2 positive or negative |
