HPV high risk mRNA

Test code

IHR

Specimen

10 mL ThinPrep

Container

ThinPrep bottle

Special instructions

Nil

Collection

Samples in cytology preservative should be collected using a cervical broom and placed in liquid-based cytology fixative

Causes for rejection

specimen more than 30 days old in liquid-based preservative; insufficient tissue

Description of analysis

This test detects oncogenic E6/E7 messenger RNA (mRNA) transcripts of 6 high-risk Human papillomavirus (HPV): subtypes 16, 18, 52, 53, 58, & 68b, which accounted for more than 76% of all cervical cancers viral mRNA in specimen

Test methodology

It is done by using reverse transcription polymerase chain reaction (RT-PCR)

Limitation

Nil

Storage instruction

Specimen should be kept at room temperature

Reference

Lie AK, et al. (2005) Gynecol Oncol. Jun; 97(3):908-15 / Epidemiologic Risk Classification of HPV type is based on International Agency for Research on Cancer Multi-center Cervical Cancer Study Group. New Engl. J. Med. (2003)