PCR for Trisomy (13, 18, 21 / X&Y)

GFU

3 mL amniotic fluid or 20 mg chorionic villi

Sterile plastic conical tube or sterile plastic conical tube with transport medium

Nil

Nil

Frozen specimen; gross contamination with red cells; non-sterile container; container with rubber stopper (rubber is toxic to amniocytes)

Multiplex PCR analysis design for screening three common chromosomal aneuploidies Trisomy 21 (Down’s syndrome), Trisomy 18 (Edward syndrome), and Trisomy 13 (Patau syndrome) plus gender determination

DNA is extracted and purified from sample and is subjected to polymerase chain reaction (PCR) amplification. The amplified products are analyzed by gel electrophoresis using genetic analyzer

Gestational age must be at least 10 weeks; fetus must be a singleton. Cases of multiple pregnancy (eg, twins), mosaicism, partial trisomy, and translocations are not suitable for this essay

Specimen should be kept at room temperature

Nil