GMY
3 mL EDTA blood
Purple-top (EDTA) tube
Nil
Nil
Frozen specimen; clotted specimen; use of improper anticoagulant; hemolysis
DNA microdeletion analysis of the three sub-regions (AZFa, AZFb & AZFc) in the azoospermia factor (AZF) loci on the long arm of the Y chromosome
DNA is extracted from sample and subjected to multiplex polymerase chain reaction (PCR) amplification. The amplified products are analyzed by agarose gel electrophoresis
The Y chromosomal microdeletion test can only detect complete ablation of the tested region. However, the possibility of mosaicism and partial deletion of the AZF loci cannot be ruled out in non-deleted situation
Specimen should be kept at 4°C
EAA/EMQN best practice guideline for molecular diagnosis of y-chromosomal microdeletions. State of the art (2004)