What is it?
Identify the D816V mutation
KIT, also called CD117 or C-Kit, is a receptor tyrosine kinase that binds to stem cell factor to facilitate the growth of blood cells.
The C-Kit D816V mutation has been implicated in the pathogenesis of several diseases including:
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Systemic mastocytosis (SM)
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Gastrointestinal stromal tumor (GIST)
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Acute myeloid leukemia (AML)
The D816V mutation also suggests a patient is unlikely to respond to C-kit-targeted treatment with imatinib and tyrosine kinase inhibitor.
DiagCor C-Kit real time PCR kit is designed to identify the D816V mutation.
Fast and cost-effective, this highly sensitive molecular technique can detect as low as 0.1% of mutations present in 99.9% wild-type DNA background.
Who is it for?
This kit is especially valuable to oncology practitioners and researchers concerned with identifying and treating various pathologies, including systemic mastocytosis (SM), gastrointestinal stromal tumor (GIST) and acute myeloid leukemia (AML).
GenoQuest C-Kit real time PCR kit is also useful to oncology researchers.
Why is it different?
This highly sensitive kit allows selective site-specific detection of mutant DNA.
It can detect less than 0.1% mutant sequence copies against a background of 99.9% wild-type DNA.
The ready-to-use reagent is compatible with various real time PCR machines and delivers results 2.5 hours after extraction.
Advantages
Accurate – Highly sensitive test allows site-specific detection of less than 0.1% mutant sequence copies in a background of 99.9% wild-type DNA
Practical – Compatible with various real time PCR instruments
Fast – Delivers results in approximately 2.5 hours after extraction
Easy – Ready-to-use reagent
Cost effective – Highly price-competitive
Need to know
This is a consumable assay kit, developed, supplied and supported by DiagCor. It requires a compatible instrument and a meticulous MDx workflow.
What you get
In the box
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One duplex (FAM + Cy5) C-Kit mutation assay with internal control for each assay
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Real time master mix
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DNase free water
Service and support
The DiagCor support team includes skilled technicians, engineers and scientists
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Distributor and end-users receive direct training supported by video conferencing
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Standard operation procedures explain test execution step-by-step
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Experienced technicians will familiarize users with procedures by guiding them through a series of trial tests
DiagCor provides extensive training to end users and distributors that covers
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Correct application of the system, including how to deploy in public health programs for best ROI
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Practical lab technician training
Equipment required
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Real time PCR machine
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High speed centrifuge
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Vortex
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Pipettes
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Water bath
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Ice bucket
Extraction kits
QIAamp FFPE extraction kit
Analytes
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C-Kit mutation (D816V)
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Internal control
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Negative control
Specs
| Catalogue number |
R14002 |
| Configuration |
24 tests (real time PCR kit) |
| Specimens |
Human genomic DNA extracted from fresh, frozen or paraffin embedded tissue. DNA must be extracted from materials prior to use and stored at -20°C. The OD value of DNA samples should be measured using a spectrophotometer after extraction |
| Internal controls |
Internal Control; NTC Control |
| Reported results |
Wildtype or C-Kit D816V mutation |
