What is it?
Identify the V617F mutation
Janus kinase 2 (JAK2) is a non-receptor tyrosine kinase which is especially important for controlling the production of blood cells from hematopoietic stem cells.
Somatic mutation V617F in JAK2 results in the production of a JAK2 protein that is constantly activated, which leads to the uncontrolled or abnormal proliferation of blood cells.
The most common conditions associated with the V617F mutation are:
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Essential thrombocythemia (ET)
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Polycythemia vera (PV)
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Primary myelofibrosis (PMF)
The GenoQuest JAK2 real time PCR kit identifies the V617F mutation quickly and cost-effectively.
Fast and cost-effective, this highly sensitive molecular technique can detect as low as 0.1% of mutations present in 99.9% wild-type DNA background.
Who is it for?
This assay is especially valuable to oncology practitioners and researchers concerned with identifying and treating various pathologies, including essential thrombocythemia (ET), polycythemia vera (PV) and primary myelofibrosis (PMF).
It is intended for in vitro diagnostic use with the appropriate clinical laboratory settings, safety standards and properly trained personnel.
It may also be useful to oncology researchers.
Why is it different?
This highly sensitive kit allows selective site-specific detection of mutant DNA.
It can detect less than 0.1% mutant sequence copies against a background of 99.9% wild-type DNA.
The ready-to-use reagent is compatible with various real time PCR machines and delivers results 2.5 hours after extraction.
Advantages
Accurate – Highly sensitive test allows site-specific detection of less than 0.1% mutant sequence copies in a background of 99.9% wild-type DNA
Practical – Compatible with various real time PCR instruments
Fast – Delivers results in approximately 2.5 hours after extraction
Easy – Ready-to-use reagent
Cost effective – Highly price-competitive
Need to know
This is a consumable assay kit, developed, supplied and supported by DiagCor. It requires a compatible instrument and a meticulous MDx workflow.
What you get
In the box
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One duplex (FAM + Cy5) JAK2 mutation assay with internal control for each assay
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Real time master mix
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DNase free water
Service and support
The DiagCor support team includes skilled technicians, engineers and scientists
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Distributor and end-users receive direct training supported by video conferencing
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Standard operation procedures explain test execution step-by-step
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Experienced technicians will familiarize users with procedures by guiding them through a series of trial tests
DiagCor provides extensive training to end users and distributors that covers
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Correct application of the system, including how to deploy in public health programs for best ROI
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Practical lab technician training
Equipment required
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Real-time PCR machine
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High speed centrifuge
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Vortex
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Pipettes
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Water bath
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Ice bucket
Extraction kits
QIAamp FFPE extraction kit
Analytes
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JAK2 mutation (V617F)
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Internal control
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NTC control
Specs
| Catalogue number |
92023 |
| Configuration |
24 tests (real time PCR assay) |
| Detected mutations |
JAK2 mutation (V617F) |
| Specimens |
Human genomic DNA extracted from fresh, frozen or paraffin embedded tissue and blood. DNA must be extracted from materials prior to use and stored at -20°C. The OD value of DNA samples should be measured using a spectrophotometer after extraction |
| Internal controls |
Internal Control; NTC Control |
| Reported results |
Positive or negative of JAK2 V617F mutation |
