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GenoScreenTM SARS-CoV-2 RT-qPCR Assay

SARS-CoV-2 RT-qPCR Assay is designed for the detection of SARS-like coronaviruses in human respiratory specimens via real time PCR analysis. It is a triplex assay that detects two viral genes (ORF1ab and N) and an internal control.

What is it?

Accurate detection of SARS-CoV-2
This sensitive, highly specific kit works with various real time PCR machines to quickly detect SARS-CoV-2 in clinical specimens. 
It is a triplex assay that detects two viral genes (ORF1ab and N) and an internal control using specific primers and fluorescent dye-labeled oligonucleotide probes. These genes are amplified and detected in commonly used FAM, HEX/VIC and Cy5 fluorescent channels, respectively.
The cost-effective ready-to-use reagent produces results two hours after extraction.

Who is it for?

This assay is intended exclusively for in vitro diagnostic use with the appropriate clinical laboratory settings, safety standards and properly trained personnel.
It is for SARS-like coronaviruses RNA detection and has been validated using human respiratory samples (e.g. deep throat saliva) extracted by a CE IVD extraction system and designated PCR platform.

Why is it different?

The design is adapted from well established HKU-Pasteur protocol with further improvement, simplifying the master mix preparation step from two monoplex assays to a single triplex assay with the combination use of highly thermostable enzymes.  
The ready-to-use reagent is compatible with various real time PCR machines and delivers results 2 hours after extraction.


Fast – Delivers results in approximately 2 hours, tests up to 96 samples simultaneously
Accurate – Reliable ready-to-use reagent
Cost effective – Lowest overall cost in market
Easy – No post-PCR manipulation required

Need to know

This is a consumable assay kit, developed, supplied and supported by DiagCor. It requires a compatible instrument and a meticulous MDx workflow.

  • Works with

    Genesis 96Q Real-Time PCR System

    Genesis 96Q Real-Time PCR System is a compact 96-well real-time PCR (qPCR) instrument with up to 6 detection channels to satisfy various analysis methods.

    Applied Biosystems ViiA7

  • Workflow
    Workflow (GenoScreen & GenoQuant)

What you get

In the box

  • 5x master mix
  • 40x RT mix
  • SARS-CoV-2 primer mix
  • Magnesium chloride
  • SARS-CoV-2 positive control
  • Negative control 
  • Nuclease-free water

Service and support
The DiagCor support team includes skilled technicians, engineers and scientists
  • Distributor and end-users receive direct training supported by video conferencing
  • Standard operation procedures explain test execution step-by-step 
  • Experienced technicians will familiarize users with procedures by guiding them through a series of trial tests 
DiagCor provides extensive training to end users and distributors that covers
  • Correct application of the system, including how to deploy in public health programs for best ROI
  • Practical lab technician training    

Equipment required
  • Real-time PCR machine
  • Centrifuge
  • Vortex
  • Pipettes
  • Water bath
  • Ice bucket 

Extraction kits

QIAamp Viral RNA Mini Kit (QIAGEN, Germany)

Internal amplification control (IAC)
SARS-CoV-2 (ORF1ab and N genes)

Catalogue number 92027
Configuration 48 tests (real time PCR kit)
Detected genes ORF1ab and N genes of SARS-CoV-2
Analytical sensitivity ≥ 10 copies/rxn
Specimens Human respiratory tract specimens
Internal controls Positive control; negative control; Internal amplification control (IAC)
Reported results SARS-CoV-2 positive or negative