DiagPuro^TM Direct Cell Lysis DNA Extraction Reagent

What is it?

The product provides a simple and fast single-tube cell lysis method for isolation of DNA for downstream analysis. The reagent contains synthetic non-ionic detergents which are used to break down cells for DNA extraction and genotyping via Polymerase chain reaction (PCR). It contains Proteinase K which digests proteins and removes contamination from nucleic acid preparations. Our proteinase K inactivates nucleases that could degrade DNA during isolation. Cell pellets can be extracted in about 15 minutes.

The steps involved include:

• Prepare and wash cell pellets
• Centrifuge and remove supernatant
• Add lysis buffer and heat
• DNA-containing supernatant is ready for downstream analysis

Who is it for?

The Direct Cell Lysis DNA Extraction Reagent is useful for MDx research labs, healthcare institutions and MDx services labs around the world.

The nucleic acid extracted with the reagent is suitable for direct downstream analysis by standard amplification methods.

Why is it different?

• Simple workflow with single-tube operation
• Ultra-low consumable requirement and low cost
• Short preparation time (cell pellets are extracted in about 15 minutes)
• Compatible with PCR amplification technologies

Easy – Simple workflow with single-tube operation

Cost effective – Ultra-low consumable requirement and low cost

Fast – Short preparation time (cell pellets are extracted in 15 minutes)

Flexible – Compatible with PCR amplification technologies

Need to know

This extraction reagent yields high quality genomic, bacterial or viral DNA from human samples, ready to use in PCR and blotting procedures.

• Workflow
  This workflow is for the Direct Cell Lysis Reagent. Please refer to the product IFU for the detailed test procedure.