The Direct Cell Lysis DNA Extraction Reagent allows the simple preparation of cell lysates from cell suspensions or isolated cells of urine and liquid-based cytology (LBC) specimen, which enables direct amplification.
The product provides a simple and fast single-tube cell lysis method for isolation of DNA for downstream analysis. The reagent contains synthetic non-ionic detergents which are used to break down cells for DNA extraction and genotyping via Polymerase chain reaction (PCR). It contains Proteinase K which digests proteins and removes contamination from nucleic acid preparations. Our proteinase K inactivates nucleases that could degrade DNA during isolation. Cell pellets can be extracted in about 15 minutes.
The steps involved include:
The Direct Cell Lysis DNA Extraction Reagent is useful for MDx research labs, healthcare institutions and MDx services labs around the world.
The nucleic acid extracted with the reagent is suitable for direct downstream analysis by standard amplification methods.
This extraction reagent yields high quality genomic, bacterial or viral DNA from human samples, ready to use in PCR and blotting procedures.
The DiagCor support team includes skilled technicians, engineers and scientists.